Abstract
Chromatin condition is crucial for the cells to respond to their environment. In C. elegans, post-embryonic development is accompanied by the exit of progenitor cells from quiescence in response to food. The chromatin protein LET-418/Mi2 is required for this transition in development indicating that proper chromatin structure in cells of the freshly hatched larvae is important to respond to food. However, the identity of the tissue or cells where LET-418/Mi2 is required, as well as the developmental signals that it is modulating have not been elucidated. By restoring the activity of LET-418/Mi2 in specific tissues, we demonstrate that its activity in the intestine and the hypodermis is able to promote in a cell non-autonomous manner the exit of blast cells from quiescence and further development. Furthermore, we identify the IIS (insulin/insulin-like growth factor signaling) pathway to be one of the signaling pathways that is conveying LET-418/Mi2 cell non-autonomous effect on development.
Highlights
Chromatin remodelers play important roles during metazoan development through their regulatory role in gene expression
None of the other transgenes promote exit from the L1 developmental arrest (Table 1). These results show that LET-418 functions cell non-autonomously and indicate that LET-418 is required for chromatin function in the intestine and to a lesser extent in the hypodermis to initiate postembryonic development
LET-418/Mi2 plays a major role in the intestine and the hypodermis for the the development of the whole organism and its impact on other tissues is mediated by the insulin development of the whole organism and its impact on other tissues is mediated by the insulin signaling pathway
Summary
Chromatin remodelers play important roles during metazoan development through their regulatory role in gene expression. In C. elegans post-embryonic development is not initiated in the absence of food and the freshly hatched L1 larvae go into a diapause stage until food is available [13]. Elegans post-embryonic development is not initiated in the absence of food and the freshly hatched L1 larvae go into a diapause stage until food is available [13] The developmental arrest associated with let-418 mutation is dependent on a network of chromatin regulators Most of these chromatin associated proteins are part of large complexes that are involved in activation of transcription [12]. These findings highlight the importance of chromatin state in cells of the freshly hatched larvae to ensure a proper response to the environment. We show that the cell non-autonomous function of LET-418 in triggering the exit of blast and germ cells from quiescence relies on the insulin signaling pathway
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