Abstract
Six near-term placentae from rhesus monkeys ( Macaca mulatta) were analyzed for their contents of choline acetyltransferase (ChAc), acetylcholine (ACh) and choline (Ch). ChAc was measured by a radiometric assay in the presence of [1- 14C]acetylcoenzyme A and choline and distinguished from other acetyltransferases by a differential assay involving acetylcholinesterase or selective ion pair extraction of [1− 14C]ACh with tetraphenylboron. At 150 days of gestation the rhesus placenta synthesized 4.067 ± 0.737 μmoles ACh/g wet weight/hr. Ch and ACh were determined with a radiochemical method based on the phosphorylation of free Ch by choline kinase in the presence of [ 32P]ATP. ACh was first isolated by ion pair extraction and high voltage electrophoresis. Tissue levels of Ch ranged from 737 to 3892 and ACh from 8.8 to 29.0 (nmoles/g wet weight). The rhesus monkey appears to be a suitable animal model to study the physiological significance of ACh in the placenta in vivo.
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