The chloroplast CF 0I subunit can replace the b-subunit of the F 0F 1-ATPase in a mutant strain of Escherichia coli K12

Abstract

The amino acid sequence of the CF 0I subunit from the chloroplast F 0F 1-ATPase has only a low similarity to the amino acid sequence of the b-subunit of the E. coli F 0F 1-ATPase. However, secondary and tertiary structure predictions plus the distribution of hydrophobic and hydrophilic amino acids have indicated that these two subunits serve a similar function. This proposition was investigated directly. A cDNA clone for the chloroplast atpF gene, encoding the CF 0I subunit, was altered by site-diected mutagenesis such that the translation start site corresponded to the N-terminus of the mature protein. An E. coli mutant strain carrying a chain-terminating mutation in the uncF gene, encoding the b-subunit, was transformed with the plasmid carrying the altered atpF gene. The resultant transformant was able to grow on succinate and gave a growth yield similar to that of a wild-type control. Assays on membrane preparations from the transformant also clearly indicated that the mature CF 0I subunit from spinach chloroplasts was able to replace the E. coli b-subunit in the E. coli F 0F 1-ATPase.