Abstract
The increase of chlorophyll fluorescence yield in chloroplasts in a 12.5 Hz train of saturating single turnover flashes and the kinetics of fluorescence yield decay after the last flash have been analyzed. The approximate twofold increase in F m relative to F o, reached after 30–40 flashes, is associated with a proportional change in the slow (1–20 s) component of the multiphasic decay. This component reflects the accumulation of a sizeable fraction of Q B-nonreducing centers. It is hypothesized that the generation of these centers occurs in association with proton transport across the thylakoid membrane. The data are quantitatively consistent with a model in which the fluorescence quenching of Q B-nonreducing centers is reversibly released after second excitation and electron trapping on the acceptor side of Photosystem II.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Bioenergetics
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