Abstract
As a preliminary to immunological and physiological studies, we have been attempting to define major biochemical and biophysical changes on the cell surface following transformation. Many of our initial comparisons were carried out with established lines (Wu et al. 1969). However, a concern in such studies is the known aneuploidy of continuous lines and the tendency toward genetic drift under the twin pressures of unstable chromosome complement and continuous passaging in vitro. These objections can be overcome by using a system in which a diploid population of cells can be rapidly converted from the normal to the transformed phenotype by use of a transforming virus and corresponding virus mutants with a temperature-sensitive transforming function. We have used the ts 68 mutant of the Schmidt-Rupin strain of Rous sarcoma virus to transform secondary chick embryo fibroblasts. Fibroblasts so infected have normal morphology at 41°C but are rapidly converted to a transformed...
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
More From: Cold Spring Harbor Symposia on Quantitative Biology
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.