The Chemotherapy-Free Combination of Vemurafenib and Rituximab Produces Deep and Durable Responses in Relapsed or Refractory Hairy Cell Leukemia (HCL) Patients

Abstract

BACKGROUNDPurine analogs are very effective in HCL, but up to 50% of patients relapse. We discovered the BRAF-V600E mutation as the genetic cause of HCL (Tiacci et al., NEJM 2011) and documented, in 26 relapsed/refractory patients, a high response rate (96%) after treatment with the BRAF inhibitor vemurafenib given for a median of 16 weeks (Tiacci, Park et al., NEJM 2015). Responses were achieved after a median of 8 weeks and included 35% complete remissions (CR) and 61% partial remissions (PR). In all cases, residual leukemia was present in the bone marrow and the median relapse-free survival was 9 months from the end of treatment. Since HCL expresses high levels of CD20, rituximab might target leukemic cells resistant to vemurafenib and improve the efficacy of BRAF inhibition in a chemotherapy-free manner.METHODSIn this academic, phase-2, single-arm, single-center trial (EudraCT 2014-003046-27), HCL patients refractory to, or relapsed after, purine analogs received vemurafenib (960 mg twice daily orally) for 8 weeks and concomitant rituximab (375 mg/m2 intravenously) every 2 weeks. Rituximab was also given four times every 2 weeks after the end of vemurafenib dosing. CR required normal blood counts, no splenomegaly, and no leukemic cells visible in the bone marrow biopsy and in the blood based on morphological criteria.RESULTSIn 27 months we enrolled 31 patients (median age: 59 years; range 35-81), who had undergone a median of 3 previous therapies (range 1-14). Eight of 31 cases (26%) were primary refractory to a purine analog and, among the remaining 23 patients, 48% (n=11) had received 4 or more prior treatments. Adverse reactions of vemurafenib plus rituximab were mostly of grade 1-2, did not include myelosuppression and were consistent with the known toxicities of either drug when used alone.Strikingly, CR was achieved by all 25 patients evaluable for efficacy (100%), including 2 patients with incomplete platelet recovery (82 x 109/l and 98 x 109/l, versus 100 x 109/l per protocol definition). These 25 patients, in addition to having all previously received purine analogs, had been also refractory to previous rituximab monotherapy (n=5) and/or had relapsed after a prior BRAF inhibitor (n=7, of whom 5 had obtained a short-lived PR and 2 a CR after the BRAF inhibitor). Notably, 16/23 evaluable patients (70%) obtained a CR after only 4 weeks of vemurafenib and two concomitant doses of rituximab. Minimal residual disease (MRD) by allele-specific PCR (limit of detection: 0.05% BRAF-V600E alleles) was absent in the bone marrow of 14/23 (65%) evaluable patients; in 8 of these 14 patients (57%), MRD clearing was obtained even before sequential rituximab dosing post-vemurafenib.In evaluable patients who already completed treatment (n=25), progression-free survival was 100% after a median of 14 months (range 5-27). Among the 14 MRD-negative cases, 10 underwent follow-up bone marrow evaluations and 9 of them (90%) maintain the MRD-negativity after a median of 14.5 months (range 3-18.5); in the remaining patient, MRD reappeared 12.5 months later. Updated results on all patients (n=31) will be presented.CONCLUSIONSVemurafenib plus rituximab represents a short, safe and non-myelotoxic regimen that produces deep and durable responses in heavily pre-treated relapsed/refractory HCL patients, and is clearly superior to historical results obtained with vemurafenib or rituximab alone. Testing this regimen in the frontline setting is warranted, and should be carried out in a randomized clinical trial against the chemotherapy-based standard of care. DisclosuresTiacci:Roche: Research Funding. Zaja:Novartis: Honoraria, Research Funding; Roche: Honoraria, Research Funding; Takeda: Honoraria; Janssem: Honoraria; Gilead: Honoraria; Abbvie: Honoraria; Celgene: Honoraria, Research Funding. Zinzani:Celgene, Roche, Janssen, Gilead, Takeda, BMS, MSD, Servier, Sandoz, Mundipharma: Honoraria; Celgene, Janssen, Gilead, Roche, Takeda, BMS, MSD, Sandoz, Servier, Mundipharma: Speakers Bureau; Merck: Consultancy, Other: Advisory board. Gaidano:Janssen: Consultancy, Honoraria; Gilead: Consultancy, Honoraria; AbbVie: Consultancy, Honoraria; Roche: Consultancy, Honoraria; Amgen: Consultancy, Honoraria. Foà:Celgene: Consultancy, Speakers Bureau; BMS: Consultancy, Speakers Bureau; Sandoz: Consultancy, Speakers Bureau; Amgen: Consultancy, Speakers Bureau; Roche: Consultancy, Speakers Bureau; Gilead: Consultancy, Speakers Bureau; Janssen: Consultancy, Speakers Bureau; Novartis: Consultancy, Speakers Bureau; Abbvie: Consultancy, Speakers Bureau. Falini:Roche: Research Funding.