The chemokine SDF-1/CXCL12 and Vasopressin expression in the hypothalamus of Tunisian rodent, Meriones shawi, subjected to water restriction and Cd exposure

Abstract

Event Abstract Back to Event The chemokine SDF-1/CXCL12 and Vasopressin expression in the hypothalamus of Tunisian rodent, Meriones shawi, subjected to water restriction and Cd exposure Sihem Mbarek1*, Tounes Saidi1, William Rostene2, Stephane Parsandaniantz2 and Rafika B. Chaouacha-Chekir1 1 Institut Superieur de Biotechnologie Sidi Thabet 2020, Laboratoire d'Ecophysiologie Generale et Comparee, Tunisia 2 INSERM-UPMC UMRS 732, Hospital Saint Antoine, France Stromal-cell-derived factor 1 (SDF-1/CXCL12) is a chemokine recently found to be constitutively expressed with it receptor in vasopressinergic neurons in the supraoptic (SON) and the paraventricular nuclei (PVN). The phasic pattern of these neurons facilitates sustained antidiuresis during dehydration. Here, we investigated whether SDF-1 can interact with arginine-vasopressin (AVP) to maintain body fluid homeostasis in a rodent adapted to desert life (Meriones shawi). In order to clarify the implication of SDF-1 in water metabolism regulation, we sought to determine whether SDF-1 colocalized with AVP in both SON and PVN in basal and water dehydration conditions. We also investigated the effects of cadmium (Cd) exposure on the co-expression of SDF-1 and AVP within these two nuclei in different water states. Using dual immunolabeling microscopy, we found that SDF-1-immunoreactive neurons colocalized with AVP within both SON and PVN magnocellular neurons in Meriones shawi. In contrast to what was observed in the laboratory rat where dehydration causes a dramatic depletion of hypothalamic AVP/ SDF-1 immunoreactivity in both SON and PVN, dehydration induced in our model an increase in expression of AVP and SDF-1. Interestingly, we observed that the ability of acute systemic dehydration to produce AVP/SDF-1 in both SON and PVN was also observed by treating Meriones with Cd. However, the co-expression of both AVP and SDF-1 immunoreactivity from dehydrated animals in the presence of Cd was markedly and significantly lower than in the absence of Cd. The present innovative data support the involvement of the chemokine SDF-1 in the control of body fluid homeostasis and suggest that changes in the expression of the SDF-1 appear to be closely linked to changes in AVP immunoreactivity. Acknowledgments: This work was supported by an INSERM-DGRSRT grant and by a grant from the University P and M Curie Paris VI Conference: 3rd Mediterranean Conference of Neuroscience , Alexandria, Egypt, 13 Dec - 16 Dec, 2009. Presentation Type: Poster Presentation Topic: Aging & Environment Citation: Mbarek S, Saidi T, Rostene W, Parsandaniantz S and Chaouacha-Chekir RB (2009). The chemokine SDF-1/CXCL12 and Vasopressin expression in the hypothalamus of Tunisian rodent, Meriones shawi, subjected to water restriction and Cd exposure. Front. Neurosci. Conference Abstract: 3rd Mediterranean Conference of Neuroscience . doi: 10.3389/conf.neuro.01.2009.16.185 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 29 Nov 2009; Published Online: 29 Nov 2009. * Correspondence: Sihem Mbarek, Institut Superieur de Biotechnologie Sidi Thabet 2020, Laboratoire d'Ecophysiologie Generale et Comparee, 2092 Tunis, Tunisia, sihemmbarek@yahoo.fr Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Sihem Mbarek Tounes Saidi William Rostene Stephane Parsandaniantz Rafika B Chaouacha-Chekir Google Sihem Mbarek Tounes Saidi William Rostene Stephane Parsandaniantz Rafika B Chaouacha-Chekir Google Scholar Sihem Mbarek Tounes Saidi William Rostene Stephane Parsandaniantz Rafika B Chaouacha-Chekir PubMed Sihem Mbarek Tounes Saidi William Rostene Stephane Parsandaniantz Rafika B Chaouacha-Chekir Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.