Abstract
The orb webs of Argiope aurantia Lucas and Argiope trifasciata (Forskal) were partitioned into three major fractions: trypsin soluble fibroin, trypsin insoluble fibroin and a water soluble fraction. The gravimetric proportions of these were nearly equal in both species. The water soluble fraction was further fractionated into KH 2PO 4, ninhydrin reactive and ninhydrin negative components. The proportions of these differed widely between the two species. The amino acid composition of the trypsin soluble fibroin and trypsin insoluble fibroin was ascertained, as well as the spinning gland luminal contents in order to assign the probable glandular origin of these fractions. The trypsin insoluble fibroin originates primarily from the large ampullate gland whereas the trypsin soluble fibroin appears to be the product of several glands.
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