The Characterization of Prokaryotic Diversity in Lake Beyşehir Using a 16s Metagenomics Study

Abstract

Lake Beysehir, located in the Central Anatolia Region, is the biggest lake in Central Anatolia, providing drinking water and irrigation. It is Turkey's third-largest lake and the largest freshwater lake. Its waters are fresh water and its depth is around 8-9 m at most. The deepest point is mentioned as 12, 14, and 15 m in different sources. Although there is a lake used by such a large population in our country, there are not enough studies on microbial diversity, so revealing the diversity is important for the lake and the population around the lake. Next-generation sequencing (NGS) research has made it possible to sequence RNA and DNA considerably quicker and cheaply than in by assessing the PCR amplicon, we utilized NGS high-throughput methods for the metagenomic research of Lake Beyşehir sequences of 16S rDNA (regions V3–V4). The analyzes revealed the phylum Proteobacteria, Verrucomicrobia, Bacteroidetes and Actinobacteria.