Abstract
AbstractMicrosatellite DNA sequences were isolated and characterized from a Puerto Rican population of communally nesting smooth‐billed anis for use in a parentage/kinship study. Lambda Zap Express (Stratagene) was used to construct a library of 350 000 independent recombinant phage and screened with TG and AAT probes. In total, 85 positive clones (73 TG and 12 AAT) were isolated. Primer pairs were designed for 17 sequences and five polymorphic loci were found (three TG and two AAT). The five loci were characterized with between four and nine alleles and heterozygosity values ranged from 0.538 to 0.840. The combined total parental exclusionary power (with neither parent known) of the five loci was 0.8869.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
