Abstract
1. 1. Adenosine deaminase from chicken serum was purified and characterized. 2. 2. The K m and V max values of the enzyme in respect of adenosine as substrate were 7.14 × 10 −3 M and 8.5 μM/min respectively. AMP, ADP & ATP are poorly metabolized respectively in decreasing order. 3. 3. The enzyme had no NA Dase and NADPase activities but the presence of either NAD or NADP in normal incubation mixture activated the enzyme. 4. 4. Metal ions Zn 2+, Ca 2+ and Mg 2+ activated the enzyme to various levels. 5. 5. Some compounds, mercaptoethanol, dithiothreitol (DTT), dithiobisnitrobenzoate (DTNB) inhibited the enzyme to various extents.
Published Version
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