Abstract
BackgroundThe primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultures.MethodsIn this study, we isolated human bone-derived cells (HBDCs) and promoted their differentiation into osteoblasts. The following parameters were evaluated: cell number and viability, total protein expression, alkaline phosphatase activity, collagenous matrix production and osteogenic genes expression, i.e., gene coding for type I collagen and alkaline phosphatase.ResultsIt was proved the results show that HBDCs intensively proliferate during the first 7 days of culture followed by differentiation accompanied by an increase in alkaline phosphatase activity. Moreover, it was observed that during the differentiation of HBDCs, the expression of integrin β1 increased.ConclusionsThe process was also accompanied by changes in cell shape and rearrangement of the actin cytoskeleton and focal contacts containing FAK and the integrin β1 subunit. We suggest that the β1 integrin subunit may be a suitable new target in studies of the differentiation of primary human osteoblasts in culture.
Highlights
The primary human bone-derived cell culture technique is used as a model to study human osteogenesis
Osteoblast differentiation from their undifferentiated to functional state is accompanied by changes in cell morphology and in the expression of adhesion molecules, extracellular matrix (ECM) proteins and specific osteogenic markers, i.e., alkaline phosphatase (ALP), osteocalcin (OC), osteopontin (OP), osteonectin (ON) and bone sialoprotein (BSP)
For a comprehensive examination of human bone-derived cells (HBDCs) in vitro, the cells were cultured under conditions that allow osteoblast differentiation
Summary
The primary human bone-derived cell culture technique is used as a model to study human osteogenesis. Compared to cell line cultures, primary osteoprogenitor and osteoblast cultures provide more complex information about osteogenesis, bone remodeling and regeneration than cell line cultures. Bone is a highly organized structure of calcified connective tissue formed during osteoprogenitor proliferation and differentiation into mature osteoblasts. Osteoblast maturation consists of three main phases: proliferation, extracellular matrix (ECM) synthesis, and mineralization [1]. Osteoblast differentiation from their undifferentiated to functional state is accompanied by changes in cell morphology and in the expression of adhesion molecules, ECM proteins (collagen type I; COLI) and specific osteogenic markers, i.e., alkaline phosphatase (ALP), osteocalcin (OC), osteopontin (OP), osteonectin (ON) and bone sialoprotein (BSP). The subsequent reconstruction of a collagenous matrix occurs in the course of procollagen I mRNA decrease [3] and OC expression increase, a late marker of differentiated osteoblasts [1]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
