Abstract
BackgroundAs one of the novel molecules, circRNA has been identified closely involved in the pathogenesis of many diseases. However, the function of circRNA in acute myeloid leukemia (AML) still remains unknown.MethodsIn the current study, the RNA expression profiles were obtained from Gene Expression Omnibus (GEO) datasets. The differentially expressed RNAs were identified using R software and the competing endogenous RNA (ceRNA) network was constructed using Cytoscape. Functional and pathway enrichment analyses were performed to identify the candidate circRNA-mediated aberrant signaling pathways. The hub genes were identified by MCODE and CytoHubba plugins of Cytoscape, and then a subnetwork regulatory module was established.ResultsA total of 27 circRNA-miRNA pairs and 208 miRNA-mRNA pairs, including 12 circRNAs, 24 miRNAs and 112 mRNAs were included in the ceRNA network. Subsequently, a subnetwork, including 4 circRNAs, 5 miRNAs and 6 mRNAs, was established based on related circRNA-miRNA-mRNA regulatory modules.ConclusionsIn summary, this work analyzes the characteristics of circRNA as competing endogenous RNA in AML pathogenesis, which would provide hints for developing novel prognostic, diagnostic and therapeutic strategy for AML.
Highlights
As one of the novel molecules, circRNA has been identified closely involved in the pathogenesis of many diseases
Acute myeloid leukemia (AML) is a type of malignant neoplasm characterized by clonal proliferation, abnormal growth and impaired differentiation of hematopoietic stem cell (HSC) in the hematopoietic system, in which immature myeloid cells infiltrate in the bone marrow, peripheral blood or other extramedullary tissue [1,2,3]
The regulated function of circRNA is mainly based on the competing endogenous RNA hypothesis which was proposed in 2011 [9]
Summary
As one of the novel molecules, circRNA has been identified closely involved in the pathogenesis of many diseases. The function of circRNA in acute myeloid leukemia (AML) still remains unknown. Noncoding RNA (ncRNA), which is characterized by non-protein coding functional RNA, plays a crucial role in AML diagnosis, prognosis and treatment [5]. The regulated function of circRNA is mainly based on the competing endogenous RNA (ceRNA) hypothesis which was proposed in 2011 [9]. The ceRNA hypothesis presents an idea that RNA transcripts communicate and regulate their respective expression levels through competing for binding microRNA response elements (MREs). CircRNA could function as miRNA sponges by binding to miRNA with MREs, which will prohibit mRNA degradation through miRNA-mediated pathways [6]. CircRNA could negatively regulates mRNA expression level as miRNA sponge by ceRNA network, and a growing number of evidences have demonstrated this hypothesis [10]. CircRNA could be a potential biomarker for diagnosis and prognosis of AML [14]
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