The Characteristics of Action Potentials in Primo-vessels and the Effects of Acetylcholine Injection

Abstract

Purpose: To investigate anti-hyperlipidemic effects of ACE against poloxamer 407(P-407)-induced hyperlipidemia of C57/BL 6 mice model. Serum and hepatic tissue lipid profiles, hyperlipidemia related gene expressions and protein levels of the hepatic tissue were measured. Methods: C57BL/6micewere orally administratedwith distilled water, Artemisia iwayomogi Kitamura(AR)50mg/kg or Curcuma longa Linne(CU) 50mg/kg, ACE(25 or 50 or 100mg/kg) or Lipitor(50mg/kg) for 13 weeks (n=10 or 11 per group). Mice were injected P-407(500mg/kg) two times in everyweeks. After 13 weeks, we measured serum lipid parameters such as total cholesterol (TC), triglycerides (TG), and hyperlipidemic gene and protein expressionswere evaluated in the heaptic tissues. Results: P-407 (500mg/kg) injection caused considerable increases of serum TC, TG and free fatty acid (FFA). The serum levels of total reactive oxygen species and hepatic tissue levels of lipidperoxidation were also increased by P-407. Treatment with ACE, however significantly normalized the above alterations. The fat accumulations were occured and accumulated in the hepatic tissue, whreas those alterations were improved by treatmentwithACEbymeasuringhistopathological inspection. Additionally, gene expression levels including SREBP-1c, FAS, SCD-1, PPARandTNFin hepatic tissuewere altered by P-407 injected, while ACE group also significantly normalized them. Conclusion: Finally, it was concluded clearly that ACE showed antihyperlipidaemic effects in P-407-induced hyperlipidaemic mice. ACE is worked in the prevention of experimental hyperlipidemia. Contact: Hyo seon Kim, khs910707@hanmail.net