The changes of β-arrestin2 and microtubule-associated protein light chain 3 in renal tissue of rats with renal ischemia reperfusion injury

Abstract

Objective To investigate the expression of β-arrestin2 and microtubule-associated protein light chain(LC)3 in renal of rat with acute renal ischemia reperfusion injury, and to analyze the relationship between them and renal injury. Methods Fifty-four male SD rat(3-4 weeks old) were randomly divided into three groups: control group, sham group, acute ischemic reperfusion injury group.We established the acute renal ischemia reperfusion injury model through removing the right kidney and clamping the left renal for 45 minutes with noninvasive arterial clip.We obtained the kidney and blood samples respectively at 12 h, 24 h, 36 h, 48 h, 72 h, 96 h after the surgery.Expressions of β-arrestin2 and LC3 protein were detected by the immunohistochemistry method and Western blot method.The renal function and morphological changes were assessed. Results Compared with control group and sham group, the serum creatinine and kidney pathological grading of acute ischemia reperfusion injury group obviously rised.The kidney injury was the most serious at the 24 h after acute ischemic reperfusion injury.The expressions of β-arrestin2 and LC3 were little in the control group and sham group.However, the expressions of these two indicators were obviously higher and reached the peak at the 12 h after acute ischemia reperfusion injury.All these results suggested that the changes of these two indicators were anterior to the histopathological changes.The expressions of β-arrestin 2 and LC3 protein were in positive correlation with the kidney injury(r=0.821, P<0.05; r=0.913, P<0.05). Conclusion In the acute renal ischemia-reperfusion injury, β-arrestin2 may be as a kind of upstream regulatory protein involving in the kidney pathological process through the regulation of the autophagy. Key words: β-arrestin2; Microtubule-associated protein light chain 3; Acute kidney injury; Ischemic reperfusion injury,