The change of F-actin and actin binding protein in intstinal epithelial cells under the ischemia and anoxia injury

Abstract

Objective To investigate the relationship between the apoptosis and the change of F-actin and actin binding protein in intstinal epithelial cells (IEC) under the ischemia and anoxia injury, and the effect of calcium channel antagonist. Methods Primary culture of rat IECs and ischemia and an-oxia injury model in vitro were established. IECs were divided into 8 groups : group A ( control group), group B (ischemia group) ,group C (anoxia group) ,group D (ischemia and anoxia group) ,group A1 ( A +2 mg/L Verapamil) ,group B1 (B +2 mg/L Verapamil) ,group C1 (C +2 mg/L Verapamil) ,group D1 ( D + 2 mg/L Verapamil). Apoptosis rate (AR) was determined by flow cytometry. F-actin, talin and α-ac-tin were observed by LSCM. Results The AR in groups B, C and D was significantly higher than in group A (P < 0.05). The AR in groups B1, C1 and D1 was lower than in groups B. C and D ( P < 0.05 ). The form and arragement of F-actin, talin and α-actin in groups B, C and D were changed with the decrease of fluorescence intensity compared with group A (P < 0.01 ), the most significant in group D. The change in groups B1, C1 and D1 was reduced and the fluorescence intensity was increased as compared with the cor-responding injury groups (P < 0.05). Conclusion The ischemia and anoxia injury results in the break-down of F-actin and actin binding protein, which is related to the apotosis of IEC. Calcium channel antago-nist can decrease AR and reduce the change of these cytoskeleton. Key words: Intestinal epithelial ceils ; Ischemia; F-actin