The change of chlorophyll fluorescence parameters in winter barley during recovery after freezing shock and as affected by cold acclimation and irradiance

Abstract

The change of chlorophyll fluorescence parameters in froze leaves of 3 leaf-age seedlings were examined using two winter barley cultivars (Chumai 1 and Mo 103) differing in cold tolerance to investigate physiological response to low temperature as affected by cold acclimation (under 3/1 °C, day/night for 5 days before freezing treatment) and irradiation size (high irradiance: 380 ± 25 μmol m −2 s −1 and low irradiance: 60 ± 25 μmol m −2 s −1) during recovery. The results showed that non-lethal freezing shock (exposed to −8 °C for 18 h) did not obviously affect maximum quantum efficiency in photosystem II (PSII), but dramatically increased non-photochemical quenching and reduced effective quantum yield in PSII. Cold acclimation significantly improved stability of photosynthetic function of leaves after freezing stress through buffering excessive energy and alleviating photoinhibition during recovery, indicating it increased recovery ability of barley plants from freezing injury. High irradiance was quite harmful to the stability of PSII in barley plants during recovery from freezing injury. The electron transport rate of PSII varied with cold-acclimation, irradiance and genotype. Cold acclimation caused significant increase in electron transport rate of PSII for relatively tolerant cultivar Mo 103, but not for relatively sensitive cultivar Chumai 1. It can be concluded that some chlorophyll fluorescence parameters during recovery from freezing shock may be used as the indicators in identification and evaluation of cold tolerance in barley.