THE CELLULAR EVALUATION OF SOME OXIDATIVE - ENDOGENOUS ENZYMATIC ANTIOXIDANTS STATUS AMONG PATIENTS WITH CUTANEOUS LEISHMANIASIS

Abstract

The current study was conducted in order to investigate the cellular-physiological responses of some enzymatic endogenous antioxidants of parasitic infection that were performed as a cellular metabolically defenses through estimation of oxidative stress biomarkers as indicators in patients with cutaneous leishmania and evaluation of these responses of endogenous antioxidants. The study included an individual of both genders: 60 patients with cutaneous leishmaniasis (CL) and 30 healthy individuals. Study volunteers were distributed into three age groups (1-20), (21- 40), and (41-60) years. The demographic data were collected for both the patient and healthy groups, including age and ABO-Rh blood groups. Also, the laboratory measurements were performed for all venous blood samples taken from patients and healthy subjects including measure the concentrations of oxidative stress biomarkers total oxidants status (TOS) and malondialdehyde (MDA), and the concentrations of enzymatic antioxidants superoxide dismutase 1 (SOD1) and glutathione peroxidase 1 (GPX1). The demographical study revealed that the incidence of CL disease more occurs in males than females within early and mid-ages. On the other hand, a higher percentage of CL incidences were an early age (1–20 years) and mid-age (21–40 years). In addition, the predominant blood type among CL patients was A+ followed by O+ > B+ >AB+ and B- > A- > O- >AB- . The serum biochemical assays of cutaneous leishmaniasis patients indicated for a significant increase in serum concentrations of (TOS) and (MDA), and a significant decrease in concentrations of SOD1 and GPX1 in comparison to the healthy group for all sample age ranges. It is concluded that the pathogenesis of cutaneous leishmaniasis caused cellular oxidative stress represented by the high production of free radicals which functionally depleted the cellular metabolically levels of serum endogenous enzymatic antioxidants.