Abstract
Five samples of tonsil, 10 reactive lymph nodes and 65 consecutive cases of non-Hodgkin lymphoma (NHL) were evaluated in suspension phenotyping with the monoclonal antibodies alpha Leu-I, alpha Leu-2a, alpha Leu-3a, OKT1, OKT3, OKT4, OKT6, OKT8, W6/32, 26/114, DA-2, 2DI, J5, AN51 and OKT9 together with conventional surface marking by rosetting (E, Fc gamma, Fc mu, C3b, C3d) and staining for surface and cytoplasmic immunoglobulin (SIg, CyIg) heavy and light chain classes. The results confirm the reproductability and specificity of staining with monoclonal antibodies against T cells and T cells subsets. Evidence is presented for reactivity of alpha Leu-I antibody with SIg positive and Ia positive cells in some lymphomas (centroblastic centrocytic, lymphocytic and immunoblastic), and 2 cases showed evidence of marking with OKT3 on SIg positive cells in T cell predominant immunoblastic lymphoma. Lymphoblastic lymphomas of T cell type expressed the marker OKT6. On the basis of these results criteria for the diagnosis of T cell lymphoma are suggested. The monoclonal antibody J5, reactive with C-ALL antigen, showed variable positivity, occasionally strong in B cells in cases of centroblastic and centrocytic follicular lymphoma. Proportions of cells staining with the monoclonal antibody OKT9 showed a correlation between levels of cellular expression of transferrin (trf) receptor and the histological grade of malignancy, OKT9+ cells being elevated in high grade lymphomas, and in some cases of transforming lymphoma of low grade histological class. These results are discussed and indicate the advantage of employing a wide range of defined monoclonal reagents in the phenotypic evaluation of NHL.
Highlights
Previous reports on phenotyping in non Hodgkin's lymphoma (NHL) indicate marked heterogeneity within tumours of similar appearance and histological class
In this paper we report the results of a phenotypic analysis of 10 reactive lymph nodes and 65 consecutive cases of NHL studied with a range of monoclonal antibodies of pan T cell and T cell subset specificity, other monoclonals of defined specificity for lymphoid cell surface antigens, and "(conventional" surface phenotyping of surface immunoglobulin bearing cells (SIg), cytoplasmic immunoglobulin bearing cells (CyIg), E rosetting cells and Fcy, Fc,u, C3b and C3d rosetting cells in the same samples
The monoclonal antibodies against T cells and T cell subsets gave equivalent results when tested against suspensions of cells from normal tonsil (Table I) and lymph nodes (Table II)
Summary
Previous reports on phenotyping in non Hodgkin's lymphoma (NHL) indicate marked heterogeneity within tumours of similar appearance and histological class. Such studies have shown broad correlation between histological class and phenotype (Jaffe et al, 1974; Gajl-Peczalska et al, 1975; Stein, 1976; Stathopoulos et al, 1977; Brouet, et al, 1977; Jaffe et al, 1977; Lukes et al, 1978; Stein et al, 1979; Habeshaw et al, 1979; Janossy et al, 1980; Aisenberg, 1981), it is not yet clear what contribution more precise immunological diagnosis can make to the management of this disease (Bloomfield et al, 1979; Strauchan et al, 1978)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.