The Cell Surface Markers Expression in Postmenopausal Women and Relation to Obesity and Bone Status.

Mira Horváthová,Kornélia Štefíková,Jana Tulinská,Zora Krivošíková,Martin Gajdoš,Michaela Szabová,Eva Jahnová,Silvia Ilavská,Viera Spustová

doi:10.3390/ijerph14070751

Mira Horváthová, Kornélia Štefíková + Show 7 more

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https://doi.org/10.3390/ijerph14070751

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Abstract

The age-related changes and hormonal deprivation in postmenopausal women are associated with the immune response alteration. The excessive fat accumulation, local and systemic inflammation may lead to dysregulation in immune function and relevant health problems, including obesity and osteoporosis. We analyzed the expression of cell surface markers in the venous blood specimens, stained with fluorophores-conjugated monoclonal antibodies and analysed by multicolour flow cytometry. The significant changes of cytotoxic, naive, and memory T-lymphocytes, plasmacytoid dendritic cells (DCs) were in postmenopausal women versus fertile women. Body mass index (BMI) affected markedly the cell surface expression of CD265/RANK. Osteoporosis is linked to reduced percentage of plasmacytoid DCs, and elevated natural Treg cells (p < 0.05). The confounding factors such as women age, BMI, bone mineral density (BMD), waist size and tissue fat affect the expression of RANK on myeloid DCs and CD40L on T-lymphocytes that might be the immunophenotypic modulators after menopause.

Highlights

The presence of immune response dysregulation is related to many health alterations in postmenopausal condition
We evaluated the expression of RANK receptor on dendritic cells (DCs) and RANKL on T-lymphocytes
We have shown that the expression of CD40L on T-lymphocytes is related to women age and waist size

Summary

Introduction

The presence of immune response dysregulation is related to many health alterations in postmenopausal condition. A general decline in immune function is observed leading to immune senescence. Several of these changes affecting postmenopausal women are gender specific. The hallmarks for immune senescence include changes in T cell ratio, memory and naive T-lymphocytes, effector T- and B-lymphocytes. Postmenopausal women show an inflammatory immune microenvironment, reduced ability to respond to pathogens or stimuli, decreased cytotoxic activity of natural killer (NK) cells, higher chronic pro-inflammatory cytokines production. The infections are more common in this group as a result of attenuated immune response and higher susceptibility to pathogenic invasion [1,2,3]

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