Abstract

We report the design, construction, and characterisation of a five-component cellular fluorescence-based thermometer and its individual parts, built from the bottom up in Escherichia coli. The sensing mechanism is based on the temperature-sensitive regulatory activity of the mutated LacIts transcription factor. Such microscale temperature sensors have potential applications in the measurement of temperature gradients on 2D and 3D contours and in microelectromechanical system (MEMS) devices.

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