The cell functional control analysis of molecular chaperone in ménière’s Disease

Abstract

Problem: To analyze the function of molecular chaperone under stress (free radical) in Ménière’s disease. Methods: We created lymphoblastoid cell line (LCL) from lymphocyte of the patient who was diagnosed Ménière’s disease. We used H2O2 (free radical) as stressor. LCL was treated for 3 hours with H2O2 and ATP. Cell viability was examined using the MTT assay. The expression of molecular chaperone at protein and mRNA levels was analyzed with western blot and RT-PCR. The interaction between molecular chaperones was considered with LiquiChip of the Qiagen Company. We extracted phosphorylated protein in order to investigate the activation of molecular chaperone. Results: The viability of Ménière’s disease’s LCL treated with H2O2 was reduced to 58% after 3 hours, while the viability was maintained by ATP treatment. ATP exerted time- and dose-dependent cell viability. In addition, molecular chaperone was induced within 30 minutes at both protein and mRNA levels after ATP treatment, while H2O2 finally induced molecular chaperone after 3 hours. HSP27 was phoshorylated after ATP treatment. In addition, ATP induced protein-protein interaction and dynamic remodeling between molecular chaperones. Conclusion: Our data suggest that the early induction and dynamic remodeling of molecular chaperones by ATP treatment protect the cell from stress in Ménière’s disease. Significance: Our results indicate the importance of molecular chaperones and ATP treatment in Ménière’s disease. Support: None reported.