The cell cycle dependence of the secretory pathway in developing Xenopus laevis

Abstract

The cell cycle during the cleavage period of the amphibian Xenopus laevis is about 30 min long and oscillates between equal periods of mitosis and interphase. At the midblastula transition (MBT) the length of interphase begins to elongate and brings about corresponding changes in the activities of cell cycle-dependent processes. In this study protein secretion and Golgi processing during embryonic Xenopus development were examined. The elongation of interphase, either during normal development or experimentally induced, resulted in an increase in the secretion of both endogenous and exogenous proteins. Secretion was found to increase linearly with the increase in interphase length, indicating that the rate of secretion was constant and was regulated by the length of interphase. M-phase arrest in embryos and oocytes produced an inhibition of protein secretion that was reversible if the cell cycle was returned to interphase. This M-phase block of the secretory pathway was found to take place between the trans Golgi compartment and the plasma membrane. The developmental increase in the function of this pathway after the MBT may affect the expression of surface and secreted proteins important for the cell-cell interactions necessary for subsequent development through gastrulation.