Abstract
The ability of blastula nuclei in various portions of the cell cycle to support the development of recipient eggs was examined. A non-nutritive saline medium was developed in which isolated blastula cells remained mitotically active. This medium also allowed for ready separation of daughter cells for nuclear transplantation. Donor nuclei were derived from mitotically synchronized populations of blastula cells, obtained by a manual isolation procedure. Nuclei taken from late portions of the cell cycle frequently induced a premature second-cleavage furrow, resulting in abnormal development. However, portions of the cell cycle that preclude the participation of the donor nucleus in normal development were not detected. The donor nuclei need not have initiated or completed the S phase, nor must they be “synchronized” with the recipient egg cytoplasm.
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