Abstract
1. No significant change with time (to 24 hours) in the cataphoretic velocity of certain mammalian red cells occurs when the cells are suspended in M/15 phosphate buffer at pH = 7.35. Neither successive washings nor standing effect a change. 2. In M/15 phosphate buffer at pH 7.35 +/- 0.03 the following order of red cell velocity has been obtained. The numbers in parenthesis are micro per second per volt per centimeter. See PDF for Structure The order, though not the absolute values, was the same in buffered isotonic dextrose. Human and rabbit cells showed similar differences when both were studied simultaneously in the serum of either. Under these conditions, there is no apparent relationship between zoological Order and cataphoretic velocity. 3. Cholesterol and quartz adsorb gelatin from dilute solution in the phosphate buffer. Red cells, on the other hand, even after 24 hours contact with gelatin solution, retain their previous velocity. 4. Pregnant and non-pregnant white female humans have the same red cell cataphoretic velocity. (The cells were not agglutinated.) 5. In a series of severe anemias no significant change in cataphoretic velocity was in general apparent, although marked changes in the morphology of the red cells were present.
Highlights
The cataphoretic velocity of the red blood cells of different mammals is determined by certain surface characteristics of the cells and is of value in studying the constitution of the cell surface
The large differences in mobility found amongst the various mammals, the speed of the rabbit cells of the order 0.55~ per second per volt per centimeter at the one extreme and the dog cells 1.65~ per second per volt per centimeter, at the other extreme, indicate that it is probably not strictly permissible to speak of a characteristic red cell surface in general
It is not intended to suggest that cholesterol is a constituent of the red cell surface but rather that substances of a somewhat similar constitution plus protein could account for the high mobility of the dog cell, with mixtures of protein plus this substance or substances producing the intermediate velocities
Summary
The cataphoretic velocity of the red blood cells of different mammals is determined by certain surface characteristics of the cells and is of value in studying the constitution of the cell surface. There have been a great many experiments dealing with the sign of charge of red cells and the order of speed with which the red cells of different mammals move in an electric field. These experiments, must be considered for the most part as qualitative, and when fine distinctions are to be drawn, very frequently erroneous. Kozawa (1) noted that the red cells of different animals could be brought to an isoelectric point at a pH characteristic for each animal, the pH decreasing in the order
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