Abstract
27-year-old case with hemoglobin (Hb) Hamadan was presented. During premarital thalassemia screening an unidentified Hb peak; retention time (RT): (5.55 min, 46.2%) was determined with cation-exchange high-performance liquid chromatography (CE-HPLC) at premier resolution (Trinity-Biotech) system. Variant Hb was eluted with Hb A2 (RT): 3.76 min, 45%) at Variant II Turbo (Bio-Rad), while it was 45.7% and detected at S zone at capillary zone electrophoresis system (CZE) Sebia (Lisses, France). She didn't show any clinical symptoms or hematological abnormality. Hemoglobin A1c (HbA1c) results by Boronate affinity, CE-HPLC and CZE methods were 5.7%, 4.7%, and 5.3% respectively. Beta globin gene sequencing revealed a heterozygote codon (c.169G>C (P.Gly57Arg) identical with Hb Hamadan. Hb Hamadan does not cause clinical complaints, but may be important in prenatal examination and cause interference in HbA1c measurements.
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