Abstract
AbstractEarlier work has shown that protochlorophyll(ide) holochrome is associated with the prolamellar body membranes in etioplasts of barley (Hordeum vulgare L.), and that this pigment‐protein complex can be extracted in a stable, photoactive form by the use of saponin. For future work it would be advantageous if saponin, a detergent mixture, could be replaced by a single, well‐characterized substance. The spectral characteristics of holochrome extracted with 10 ionic and nonionic detergents were compared to those of the holochrome extracted with saponin. Mulgofen BC‐840 and digitonin extracted significant amounts of photoactive protochlorophyll(ide) holochrome, but this activity was highly labile, and no adequate substitute for saponin was found. Thus the stabilizing and solubilizing function of saponin is not simply related to the general properties of detergents.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
