The canine isolate Lactobacillus acidophilus LAB20 adheres to intestinal epithelium and attenuates LPS-induced IL-8 secretion of enterocytes in vitro.

Justus Reunanen,Veera Kainulainen,Per Saris,Reetta Satokari,Thomas Spillmann,Yurui Tang,Susanne Kilpinen

doi:10.1186/s12866-014-0337-9

Abstract

BackgroundFor a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The strain Lactobacillus acidophilus LAB20 was isolated from the jejunum of a healthy dog, where it was found to be the most pre-dominant lactobacilli. In this study, the adhesion ability of LAB20 to intestinal epithelial cell (IECs) lines, IECs isolated from canine intestinal biopsies, and to canine, porcine and human intestinal mucus was investigated. Further, we studied the ability of LAB20 to fortify the epithelial cell monolayer and to reduce LPS-induced interleukin (IL-8) release from enterocytes.ResultsWe found that LAB20 presented higher adhesion to canine colonic mucus as compared to mucus isolated from porcine colon. LAB20 showed adhesion to HT-29 and Caco-2 cell lines, and importantly also to canine IECs isolated from canine intestinal biopsies. In addition, LAB20 increased the transepithelial electrical resistance (TER) of enterocyte monolayers and thus strengthened the intestinal barrier function. The strain showed also anti-inflammatory capacity in being able to attenuate the LPS-induced IL-8 production of HT-29 cells.ConclusionIn conclusion, canine indigenous strain LAB20 is a potential probiotic candidate for dogs adhering to the host epithelium and showing intestinal barrier fortifying and anti-inflammatory effects.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-014-0337-9) contains supplementary material, which is available to authorized users.

Highlights

For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain
Adhesion to mucus First, we studied the ability of L. acidophilus LAB20 to bind mucus isolated from duodenum, jejunum, ileum, cecum and colon of canine intestine
We found that LAB20 showed very similar adhesion efficiency to canine mucus despite the mucus type i.e. from which intestinal compartments the mucus was isolated from (Figure 1A)

Summary

Introduction

For a good probiotic candidate, the abilities to adhere to intestinal epithelium and to fortify barrier function are considered to be crucial for colonization and functionality of the strain. The adhesion ability of LAB20 to intestinal epithelial cell (IECs) lines, IECs isolated from canine intestinal biopsies, and to canine, porcine and human intestinal mucus was investigated. The mammalian gastrointestinal tract (GIT) is colonized by a complex microbiota, which interacts with the host mucosa and maintains mucosal homeostasis in healthy individuals [1,2]. The maintenance of intestinal immune and physiological homeostasis in mammals is mediated by sophisticated interaction between intestinal microbiota and the host mucosa, which consists of the epithelial cell layer and Adhesion is considered as a crucial step for intestinal bacteria to colonize and further interact with the host epithelium and immune system. Lactobacillus species can be found along the mammalian GIT with various counts, typically

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Conclusion