The Campylobacter jejuni RacRS two-component system activates the glutamate synthesis by directly upregulating γ-glutamyltranspeptidase (GGT).

Anne-Xander Van Der Stel,Paweå‚ ÅAniewski,Elå¼Bieta K Jagusztyn-Krynicka,Marc M S M Wã¶Sten,Jos P M Van Putten,Andries Van Mourik

doi:10.3389/fmicb.2015.00567

Anne-Xander Van Der Stel, Paweå‚ ÅAniewski + Show 4 more

Open Access

https://doi.org/10.3389/fmicb.2015.00567

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Abstract

The highly conserved enzyme γ-glutamyltranspeptidase (GGT) plays an important role in metabolism of glutathione and glutamine. Yet, the regulation of ggt transcription in prokaryotes is poorly understood. In the human pathogen Campylobacter jejuni, GGT is important as it contributes to persistent colonization of the gut. Here we show that the GGT activity in C. jejuni is dependent on a functional RacRS (reduced ability to colonize) two-component system. Electrophoretic mobility shift and luciferase reporter assays indicate that the response regulator RacR binds to a promoter region ~80 bp upstream of the ggt transcriptional start site, which contains a recently identified RacR DNA binding consensus sequence. RacR needs to be phosphorylated to activate the transcription of the ggt gene, which is the case under low oxygen conditions in presence of alternative electron acceptors. A functional GGT and RacR are needed to allow C. jejuni to grow optimally on glutamine as sole carbon source under RacR inducing conditions. However, when additional carbon sources are present C. jejuni is capable of utilizing glutamine independently of GGT. RacR is the first prokaryotic transcription factor known to directly up-regulate both the cytoplasmic [glutamine-2-oxoglutarate aminotransferase (GOGAT)] as well as the periplasmic (GGT) production of glutamate.

Highlights

The enzyme γ-glutamyltranspeptidase (GGT, EC 2.3.2.2) is highly conserved among eukaryotic and prokaryotic organisms (Ong et al, 2008), where it has a key function in glutathione metabolism
The highly conserved enzyme GGT in prokaryotes plays a key role in the glutamine and glutathione metabolism
The ggt gene was not identified as part of the RacR regulon as the micro-arrays used in that study were based on a C. jejuni strain that lacks the ggt gene

Summary

Introduction

The enzyme γ-glutamyltranspeptidase (GGT, EC 2.3.2.2) is highly conserved among eukaryotic and prokaryotic organisms (Ong et al, 2008), where it has a key function in glutathione metabolism. In prokaryotes GGT is produced as a proenzyme in the cytoplasm and is translocated into the periplasm where it undergoes autocatalytic cleavage. This proteolysis yields a mature dimer which transfers γ-glutamyl moieties from extracellular glutathione and related compounds to amino acids or peptides or catalyzes the hydrolysis of the glutamyl group to generate glutamate (Hanigan, 1998). In Helicobacter pylori expression of GGT is reported not to be growth phase dependent, but is up-regulated at low pH (Wachino et al, 2010) and to be involved in acid resistance and immune stimulation (Gong et al, 2010; Miller and Maier, 2014)

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