Abstract
The operation of a commonly available (Phywe) type of air-driven ultracentrifuge is discussed, with particular stress upon those experimental aspects which affect the accuracy of derived sedimentation constants. The use of a thermistor in a simple bridge circuit and its calibration by the melting of diphenyl ether to give the actual temperature of the ultracentrifuge cell is described. The derived correction is found to be constant over a considerable range of values of the rotational speed and of hydrogen pressure, but it is strongly dependent upon rotor-thermistor distance. There is good evidence that the thermistor keeps pace with changing rotor temperature. A considerable measure of agreement between the sedimentation constants derived on the basis of the above calibration with those obtained from Spinco values after correction for the adiabatic cooling of the rotor is shown. Attention is drawn to the use of a ball bearing in the base of the rotor which is the probable cause of the unduly large increase in rotor temperature during normal operation.
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