Abstract
Coffee beverages are widely consumed by Indonesian, however, some of them encounter the adverse effect after drinking coffee, such as difficulty to sleep, arrhythmia and pain in the stomach. This work was aimed at determining the caffeine content of coffee beverages that are commercialized in Indonesia, covering coffee powders, coffee instants and brewed coffees. Samples were collected from the market and coffee shops. The caffeine from the samples were extracted followed by fractionation using liquid-liquid extraction with ethyl acetate. Caffeine presence in the ethyl acetate fraction was then separated on TLC and the quantity analyzed using TLC scanner. Twenty coffee samples were analyzed and the caffeine contents were ranging from 13 mg to 159 mg per-serve. Caffeine in coffee instants were approximately 50 mg per-serve, while some products of coffee powders and brewed coffees higher than 100 mg per-serve. The level of caffeine in the coffee beverage distributed in Indonesia is still below the maximal recommended level in various countries.
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