Abstract
Abstract 1. 1. Evidence is presented showing that the burst in ATP synthesis, occuring on addition of ADp to rat-liver mitochondria supplemented with β-hydroxybutyrate and phosphate, is due to the oxidation of endogenous NADH and not to a “discharg” of high-energy intermediates of oxidative phosphorylation. 2. 2. The ATP burst is about 3 times the amount of NAD + formed on the addition of ADP. 3. 3. Accompanying the ATP burst and the oxidation of endogenous NADH there is an extra oxygen uptake equivalent to the oxidation of the NADH. 4. 4. The ATP burst was abolished by 15 μM dinitrophenol. 5. 5. With succinate as substrate, the ATP burst equalled the amount of NADH oxidized, indicating that reducing equivalents from NADH compete for the respiration chain with those coming from succinate.
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