The brown algae: Sargassum binderi sonder shows a potential nephroprotective activity in in-vivo experimental model

Abstract

This study aimed to investigate the protective effect of ethanolic (EtOH) and water (H2O) extracts of brown seaweed named Sargassum binderi (S. binderi) against cisplatin (anticancer drug) - triggered nephrotoxicity. This drug is normally used for the treatment of solid tumors that causes acute kidney damage after assemblage in the renal tubules. It was an acute study, animals were divided into different groups randomly, cisplatin (7mg/kg i.p.) and normal saline were used as positive and negative control respectively. The EtOH and H2O extracts of S. bindari were given orally in a dose of 200mg/kg for 5 days to their respective groups. Nephroprotective and antioxidants potential of extracts were investigated in term of kidney and liver biomarkers, electrolytes, glucose & lipid profile along with reduced glutathione, catalase and malondialdehyde. GC-MS was performed to evaluate the presence of important therapeutic compounds in hexane extract of S.binderi. The EtOH and H2O extracts were found effective in decreasing the kidney and liver biomarkers with glucose and lipid profile, normalizing electrolytes which were disturbed by cisplatin and up-grading the antioxidant parameters by reducing the oxidative stress. GC-MS evaluation of hexane extract revealed the presence of 21 new compounds from S.binderi. Of which, 8-heptadecene, methyl tetradecanoate, 3,7,11,15-tetramethyl-2-hexadecen-1-ol, hexadecanoic acid, methyl ester were reported as antioxidant, anti-inflammatory, anti-diuretic and hypocholesterolemic compounds. S. binderi showed nephroprotective effect by stabilizing its associated biochemical parameters probably due to the antioxidant activity as confirmed by the presence of compounds.