The Brain–Pituitary–Gonadal Axis of Female Rainbow Trout Oncorhynchus mykiss: Effects of Photoperiod Manipulation1

Abstract

Two groups of post-spawned female rainbow trout were exposed to two different photoperiods, an ambient photoperiod (56°N) and a combination of long and short photoperiods (a constant 18L:6D from February 1 until May 10, then a constant 6L:18D), which acted to advance maturation and spawning. The stimulatory long–short photoperiod advanced spawning by 3–4 months and correspondingly advanced peaks in serum levels of 17β-estradiol, testosterone, calcium (an index of vitellogenin), and GTH II. Earlier events in gonadal recrudescence appeared to be less affected by the photoperiod. The initiation of exogenous vitellogenesis coincided with high levels of both pituitary salmon gonadotropin-releasing hormone (sGnRH) content and serum follicle-stimulating hormone (FSH, GTH I) levels. High levels of serum FSH were associated with rapid gonadal growth in the fish exposed to the stimulatory long–short photoperiod. In contrast, the fish exposed to the ambient photoperiod showed gonadal steroid production, formation of vitellogenin, and secondary oocyte growth without any detectable increase in serum FSH levels. The possible roles and interactions of sGnRH, gonadotropins, and steroids with respect to normal and artificially stimulated ovarian maturation are discussed.