Abstract
Marine diatoms are promising candidates for biotechnological applications, since they contain high-value compounds, naturally. To facilitate the production of these compounds, stress conditions are often preferable; however, challenges remain with respect to maximizing a metabolic potential for the large-scale cultivation. Here, we sequenced the transcriptome of diatom Skeletonema dohrnii under the actual (21 °C, 400 ppm) and elevated (25 °C, 1000 ppm) temperature and pCO2 condition. Results indicated that cells grown at higher temperature and pCO2 showed increasing growth rate, pigment composition, and biochemical productivity as did the expression of chlorophyll, carotenoid and bioactive compound related genes or transcripts. Furthermore, performing de novo transcriptome, we identified 32,884 transcript clusters and found 10,974 of them were differentially expressed between these two conditions. Analyzing the functions of differentially expressed transcripts, we found many of them involved in core metabolic and biosynthesis pathways, including chlorophyll metabolism, carotenoid, phenylpropanoid, phenylalanine and tyrosine, and flavonoid biosynthesis was upregulated. Moreover, we here demonstrated that utilizing a unique bio-fixation ability, S. dohrnii is capable of suppressing central carbon metabolism to promote lipid productivity, fatty acid contents and other bioactive compounds under high temperature and pCO2 treatment. Our study suggests that this S. dohrnii species could be a potential candidate for wide-scale biotechnological applications under elevated temperature and CO2 conditions.
Highlights
In recent years, an increasing consideration has been devoted to the possibility of growing microalgal culture for commercial purposes
We first analyzed the growth rate of S. dohrnii grown at LC and HC conditions (Table 1)
Results show that cells treated with HC condition notably increased Dissolved Inorganic Carbon (DIC), but decreased pH and CO3 −2
Summary
An increasing consideration has been devoted to the possibility of growing microalgal culture for commercial purposes. These interests are due to microalgal natural sources for wide-scale biotechnological applications including human food [1], animal feed [2], cosmetics [3], drugs [4], health products [5], biodiesel [6], fertilizers [7] and wastewater treatment [8]. To mitigate the anthropogenic CO2 concentrations with alternative renewable energy and other valuable products [9], scientists are focusing on the choice of cultivating microalgal strain, which could tolerate higher CO2 and simultaneously produce bioactive compounds and natural products [10,11].
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
