Abstract
THE BIOSYNTHESIS OF SHIKIMIC ACID FROM d-GLUCOSE
Highlights
MethodsMut,ant 83-24 of Escherichia coli, which accumulates Shikimic acid (SA) [1, 13], was grown in a medium containing labeled glucose as the sole carbon source
Acetate and bicarbonate were not significantly incorporated into Shikimic acid (SA) (Table I). Since these additions to the medium are known to be well incorporated by E. coli into products of the tricarboxylic cycle and of pyruvate, even in the presence of glucose [18], it follows t,hat the derivation of S-2,1,7 from glycolysis does not proceed through pyruvate
Shikimic acid (SA), an intermediate in the biosynthesis of several aromatic compounds, has been isolated from bacterial cultures grown on glucose variously labeled with Cl4 and on unlabeled glucose plus labeled bicarbonate, formate, acetate, or pyruvate
Summary
Mut,ant 83-24 of Escherichia coli, which accumulates SA [1, 13], was grown in a medium containing labeled glucose as the sole carbon source. Various other labeled compounds were used together with unlabeled glucose. The degradation procedure yields S-2, S-4, and S-7 individually.’. The activity of S-4 is available as the difference between SA and aconitic acid. S-l can be obtained from Reaction Sequence 3, Fig. 1, as (S-l ,7) (S-7), and S-3, when highly active relative to S-l ,7 and S-2, can be obtained by subtracting these two values from S-7,1,2,3
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