Abstract

An enzyme from wheat germ which catalyzes the formation of glucosides from phenols and uridinediphosphate glucose has been partially purified. With hydroquinone as substrate, arbutin was found to be formed as follows: UDPG + hydroquinone → UDP + arbutin Arbutin was identified by paper chromatography in several solvents either directly or after hydrolysis with acid or β-glucosidase. The rate of reaction with different phenols decreased in the order: hydroquinone > hydroxyhydroquinone > methoxyhydroquinone > resorcinol > pyrogallol > pyrocatechol.

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