Abstract
Method to detect the incorporation of 18O and 2H by isotope shift in 13C-NMR spectroscopy was applied to study the mechanism of patulin biosynthesis in Penicillium patulum NRRL 2159A. From the results of incorporation experiments with 18O2 and [1- 13C, 18O2]-acetate, monooxygenase appeared to be involved in the ring cleavage of an aromatic intermediate. 2H from [2-13C, 2-2H3]-acetate was detected at C-5, but not at C-1, indicating that the side chain protons of the aromatic intermediate were lost in the course of patulin biosynthesis.
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