Abstract
Abstract An enzymatic method for the preparation of ceramide-Glc-Gal(NeuAc)2-GalNAc (Gd2) from ceramide-Glc-Gal-(NeuAc)2-GalNAc-Gal (Gd1b) is described. Gd2, prepared in this manner, is an acceptor of galactose from UDP-Gal when incubated with a particulate subcellular fraction from rat brain to form Gd1b. This reaction was studied and compared with an analogous reaction for the synthesis of ceramide-Glc-Gal(NeuAc)-GalNAc-Gal (Gm1) using ceramide-Glc-Gal(NeuAc)-GalNAc (Gm2) as acceptor of galactose. The results obtained in this comparative study suggest that Gd2 is an intermediate in the pathway of synthesis of Gd1b. Furthermore, the transfer of galactose in the two reactions appears to be catalyzed by the same enzyme.
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