Abstract
Maximum production of 3-nitropropanoic acid by Penicillium atrovenetum occurs when the culture medium contains an auxiliary carbon source and ammonium ion. The most suitable carbon sources are dicarboxylic acids such as fumaric, succinic, and tartaric. Aspartic acid is presumably deaminated prior to its utilization in the biosynthesis. The addition of an oxidized form of ammonia to fumarate is proposed as a possible first step in the biosynthetic sequence.
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