Abstract
Incubation of homogenized head kidney tissue of the cod (Gadus morhua) and the haddock (Melanogrammus aeglefinus) with tritiated corticosterone resulted in the formation of small amounts of 18-hydroxycorticosterone (cod 0.04%, haddock 0.012% transformation per 6 g head kidney tissue per 4 h), in the presence of excess NADPH. Incubation of European eel (Anguilla anguilla) posterior vein and head kidney "mitochondria" with corticosterone-4-14C, in the presence of excess substrate and NADPH, also gave rise to 18-hydroxycorticosterone (fresh-water-adapted animals, 0.13% per mitochondria equivalent to 756 mg tissue per 2 h; animals adapted to sea water for 48 h, 0.09% per mitochondria equivalent to 456 mg tissue per 2 h). The biosynthetic, isotopically labelled 18-hydroxycorticosterone was identified by serial oxidation. Head kidneys of both the cod and haddock transformed corticosterone to cortisol. However, the unequivocal presence of aldosterone could not be shown in any of these experiments.
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