The biopreservative properties of Metschnikowia pulcherrima LMA 2038 and Trichosporon asahii LMA 810 in a model fresh cheese, are presented

Abstract

The diversity of microorganisms present in cheeses contributes to their typicity and organoleptic properties. Inhibitory strains found in these ecosystems could be used as biopreservative agents in order to replace chemicals, which are increasingly decried by consumers. Here, we isolated from cheese two yeasts named, Metschnikowia pulcherrima LMA 2038 and Trichosporon asahii LMA 810, which are active against fungi, such as Yarrowia lipolytica LMA 800, Rhodotorula mucilaginosa LMA 808, Cladosporium cladosporioides LMA 31, and Penicillium commune LMA 72, as well as against bacteria, such as Listeria innocua ATCC 33090 and Clostridium tyrobutyricum LMG 1285. When cultivated on model fresh cheese, these antagonistic yeasts significantly reduced the number of Y. lipolytica LMA 800 and R. mucilaginosa LMA 808. Preliminary data enabled us to establish a snapshot of the molecules responsible for these antifungal and antibacterial inhibitions. The molecules presumed to be present in the culture supernatant were subjected to various treatments, including heat, pH variations and enzymatic treatments. Thus, we hypothesize that different antimicrobial compounds were produced by the aforementioned antagonistic yeasts, and these molecules act in a target strain-specific manner. In summary, our findings demonstrate that cheeses possess an innumerable supply of antagonistic microbial strains, which can be exploited in the field of biopreservation to substitute chemicals and extend the shell-life of food products, including cheese.