The biomechanics of locomotor compensation after peripheral nerve lesion in the rat

Abstract

Functional recovery in animal models of nervous system disorders commonly involves behavioural compensation, in which animals alter the use of their limbs after injury, making it difficult to distinguish ‘true’ recovery from substitution of novel movements. The purpose of this study is to investigate how abnormal movements are produced by using biomechanical assessment of limb joint motion, an approach commonly used to diagnose human pathological gait. Rats were trained to cross a runway whilst kinetic (ground reaction forces) and kinematic (limb segment positions) data were synchronously recorded. Inverse dynamic analysis was used to calculate limb joint moments, or torques, and joint mechanical power throughout the stride for major joints of the forelimbs and hindlimbs, both before and after denervation of a major ankle extensor muscle. Before surgery, rats moved with joint moment and power profiles comparable to other quadrupeds, with differences attributable to species variation in limb posture. After surgery, rats trotted asymmetrically, with a near plantigrade stance of the left hindlimb. Surprisingly, ankle joint moments and power were largely preserved, with dramatic reductions in range of motion and joint moments at the proximal joints of the affected limb. Stiffening of the proximal limb compensated for increased compliance at the ankle but decreased the total mechanical work done by the injured limb. In turn, more work was done by the opposite, i.e. uninjured, hindlimb. This is the first study to quantify the biomechanical adjustments made within and between limbs in laboratory rodents after nervous system injury.