Abstract
Abstract Application of hydrostatic pressure of 15 to 25 MPa was found to cause arrest of the cell cycle in G 1 phase in the yeast Saccharomyces cerevisiae , whereas a pressure of 50 MPa did not. We found that a high concentration of L-tryptophan or a plasmid carrying the TAT2 gene which encodes a high-affinity tryptophan permeanse enabled the cells to grow at pressures of 15 to 25 MPa. Hydrostatic pressure significantly inhibited tryptophan uptake into the cells, and the ability of the uptake was impaired during incubation of the cells at 25 MPa. The activation volume associated with overall tryptophan uptake was found to be a large positive value, 46.2±3.85 ml/mol, indicating that there was a net volume increase in a rate-limiting step in tryptophan import. The result suggest that the most pressure sensitive process in terms of cell growth is tryptophan uptake, and increasing tryptophan availability enables the cell to grow under high-pressure conditions.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
