The Biocompatibility of the Root Ccanal Sealer of Primary Tooth

Abstract

An ideal temporary root canal sealer for primary teeth should be resorbable and biocompatible. It has been reported that some left over root canal sealers can cause eruption of the permanent tooth. The purpose of the present study is to evaluate the biologic effect of the root canal sealers of primary tooth. The survival rates of calcium hydroxide based and zinc oxide eugenol root canal sealer were tested in cultured U2OS cells by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl tetrazolium bromide) assay. DNA fragmentation assay was used to evaluate the toxic mechanism. The result showed that the survival rate of Vitapex fresh extract and 24-hour extract were found to have dose-dependent decreases (p＜0.05). The survival rate of Tubli seal fresh extract was found to decrease dose-dependently (p＜0.05). The survival rate of Canals fresh and 24 hour extracts were found to decrease dose-dependently (p＜0.05). DNA fragmentation showed that fresh extract and 24 hour extract group of the sealers showed had DNA cleavage that appeared as ladder patterns on electrophoresis gel. The primary tooth root canal sealers can cause U2OS cell toxicity and induce apoptosis. To prevent the alteration or interference of the permanent tooth eruption, one should avoid overuse of sealer or leaving too much sealer on the apical tissue.