The Biochemical Confirmation of Salmomella and Listeria Pathogen Microorganisms from Food with the Help of Vidas and Vitek Rapid Analyzers

Abstract

Listeria monocytogenes is widely spread in the environment and represents a potential risk when non-processed, partially processed of fermented food is consumed.
 The VIDAS L. monocytogenes II test is a rapid screening test and represents an alternative to the long-term alternative identification. This test can be used for the direct screening of L. monocytogenes in the food samples and the environment.
 Salmonella is one of the main causes of food toxic infection. Salmonella organisms are ubique in the environment and reach the intestine of humans and other mamals, especially domestic and predomestic (rodents), birds, reptiles and even insects.(Ancuţa M. Rotar, S. Apostu, 2009).
 Detecting Salmonella using classical methods: preinrichment steps, enrichment, isolation and confirmation can last up to 5 days. The screening techniques, based on the imunoenzimatic analysis (IEA) oferring the possibility of simplifying and the accelerating the detection.
 This way, the automatic IEA Salmonella VIDAS test (SLM) for detecting Salmonella in food samples and environment uses a mixture of capture antibodies with high specificity both against O antigens and H antigens, which allows the detection of both mobile and imobile Salmonella.
 The VITEK 2 GP and GN cards were developed to satisfy the growing demand for quality control measures within the food industry and provide identification of organisms in just hours, while traditional methods take days to deliver results. The GN card provides automatic identification of the most significant fermenting and non-fermenting Gram -negative bacilli, including several Salmonella species and E. coli O157, in addition to the select agent organisms Brucella melitensis, Francisella tularensis, Burkholderia mallei, Burkholderia pseudomallei and Yersinia pestis. The VITEK 2 GP card provides rapid identification of common Gram - positive organisms, including Listeria and Staphylococcus species (bioMérieux Industry).