The biochemical and molecular markers of Cyprenus carpio L. after chronic exposure to lead

Abstract

This study was designed to detect the effects of lead on biochemical and molecular characteristics of common carp, Cyprenus carpio L. Two concentrations (10 and 20 ppm) were selected for lead respectively in one week. Biochemical markers such as acetylcholinesterase, superoxide dismutase and catalase were used to assess lead exposure cytotoxic effects, while lead-induced DNA damage was used to determine the effect of lead on molecular level. Fish samples were treated with two selected concentrations of lead solution (10 and 20 ppm) for one-week duration, as exposure period, to detect the impacts of lead exposure on this species. Various biochemical markers such as acetylcholinesterase, superoxide dismutase, and catalase were applied to determine the cytotoxic impacts of lead exposure at the cellular level, while the lead-induced DNA damages were identified to reveal the influences of lead exposure at the molecular level. The results represent that the highest concentration of lead solution (20 ppm) had more effects on the antioxidant enzymes activities such as superoxide dismutase (SOD) level (655.17 ± 21.76 mg U-1) followed by acetyl cholinesterase and catalase (655.17 ± 21.76 U L-1 and 87.93 ± 7.22 mg U-1) respectively. The DNA damages were estimated by the Comet assay technique and the highest level of DNA damages were documented when lead concentration was 20 ppm using different parameters of Comet assay technique such as Comet length, tail length, and tail moment (4021 ± 56.11 µL, 523 ± 55.80 µL, and 91.208 ± 9.45 µL respectively). Therefore, this study confirms that the exposure to high lead concentrations would cause harmful effects on aquatic organisms at both cellular and molecular levels.