Abstract
3-Hydroxypropionaldehyde (3-HPA, reuterin) is a broad-spectrum natural antimicrobial agent used in the food industry and other fields. The low yield from the industrial production of 3-HPA using Lactobacillus reuteri and the spontaneous conversion of 3-HPA to acrolein have limited its more widespread use. We isolated L. reuteri BR201 as a biocatalyst for 3-HPA production and confirmed the effect of each factor in the two-step procedure for 3-HPA bioconversion. After initial cultivation for 8 h (late exponential phase), this isolate produced 378 mM of 3-HPA in 1 h at a concentration of OD600 nm 100, 30 °C, and an initial glycerol concentration of 500 mM. This is the highest reported biocatalytic yield of 3-HPA from a glycerol aqueous solution without additives. We confirmed that 4 mM of 3-HPA had antimicrobial activity against five pathogens. The degradation of 3-HPA to acrolein was greater at high temperatures, and there was little degradation when 3-HPA was maintained at 4 °C for 4 weeks. Our results may be useful for future applications of 3-HPA.
Highlights
We identified several potential lactic acid bacteria (LAB) isolates from infant feces based on color, using MRS agar containing 20 g/L glycerol and 0.3 g/L bromocresol purple
Our results demonstrated that when the cell version occurred at all tested cell density (OD600 nm 20~200) under the basic bioconversion density was high, the amount of 3-HPA decreased because the bioconversion of 1,3-PDO
Our results demonstrated that when the cell density was high, the amount of 3-HPA decreased because the bioconversion of 1,3-PDO increased
Summary
Academic Editors: Baiyu Zhang, Bo. Publisher’s Note: MDPI stays neutral with regard to jurisdictional claims in published maps and institutional affiliations. 3-Hydroxypropionaldehyde (3-HPA, reuterin), which has hydroxy and aldehyde functional groups, has many industrial uses and can be used as a platform for the synthesis of acrolein, 3-hydroxypropionic acid (3-HP), 1,3-propanediol (1,3-PDO), malonic acid, acrylamide, and acrylic acid [1,2,3]. The food industry [4,5] and healthcare industry [1] use
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