Abstract
This paper determines the optimal conditions for the directed biocatalytic oxidation of β-sitosterol by actinobacilli of the genus Rhodococcus to compounds claimed in the pharmaceutical practice: stigmast-4-en-3-one, androst-4-ene-3.17-dione, and androsta-1.4-diene-3.17-dione. Rhodococci were ascertained to convert approximately 98.0% of β-sitosterol into stigmast-4-en-3-one in the presence of n-hexadecane. The cells of rhodococci preliminarily adapted to β-sitosterol catalyze the reaction of the removal of the aliphatic lateral β-sitosterol chain with the formation of 31.5% of androstan derivatives in the presence of glucose and 2.2′-dipyridyl that is the inhibitor of 9α-hydroxylase. It was discovered for the first time that cells of R. ruber catalyzed the reaction of sterol acylation with the formation of β-sitosterol acetate along with oxidative processes.
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