The Bioactivity Prediction of Peptides from Tuna Skin Collagen Using Integrated Method Combining In Vitro and In Silico.

Liza Devita,Hanifah Nuryani Lioe,Maggy T Suhartono,Mala Nurilmala

doi:10.3390/foods10112739

Liza Devita, Hanifah Nuryani Lioe + Show 2 more

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https://doi.org/10.3390/foods10112739

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Abstract

The hydrolysates and peptide fractions of bigeye tuna (Thunnus obesus) skin collagen have been successfully studied. The hydrolysates (HPA, HPN, HPS, HBA, HBN, HBS) were the result of the hydrolysis of collagen using alcalase, neutrase, and savinase. The peptide fractions (PPA, PPN, PPS, PBA, PBN, PBS) were the fractions obtained following ultrafiltration of the hydrolysates. The antioxidant activities of the hydrolysates and peptide fractions were studied using the DPPH method. The effects of collagen types, enzymes, and molecular sizes on the antioxidant activities were analyzed using profile plots analysis. The amino acid sequences of the peptides in the fraction with the highest antioxidant activity were analyzed using LC-MS/MS. Finally, their bioactivity and characteristics were studied using in silico analysis. The hydrolysates and peptide fractions provided antioxidant activity (6.17–135.40 µmol AAE/g protein). The lower molecular weight fraction had higher antioxidant activity. Collagen from pepsin treatment produced higher activity than that of bromelain treatment. The fraction from collagen hydrolysates by savinase treatment had the highest activity compared to neutrase and alcalase treatments. The peptides in the PBN and PPS fractions of <3 kDa had antidiabetic, antihypertensive and antioxidant activities. In conclusion, they have the potential to be used in food and health applications.

Highlights

Bioactive peptides are protein fragments that provide nutrition and positive benefits for body functions [1]
Amylase, and free and total protease activity levels were determined for each sample in accordance with procedures specified by the Fédération Internationale Pharmaceutique (FIP) and the European Pharmacopoeia (PhEur)
Collagens used for the preparation of hydrolysates and peptide fractions in this study were collagens obtained from the extraction process of bigeye tuna (Thunnus obesus) skin using dilute acetic acid (0.5 M) containing bromelain and pepsin

Summary

Introduction

Bioactive peptides are protein fragments that provide nutrition and positive benefits for body functions [1]. These peptides can function as antidiabetic, antihypertensive, antimicrobial, antioxidant and others [2,3,4]. Their functionalities are influenced by the type, composition, and amino acid sequence of the peptides [5]. Bioactive peptides can have multifunctional properties, namely peptides that have more than one bioactive property [6,7,8].

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